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Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
DOI:

05:58 min

July 17, 2019

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Capítulos

  • 00:04Título
  • 00:56Saccharomyces cerevisiae Preparation
  • 02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
  • 02:51Data Analysis
  • 03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
  • 05:26Conclusion

Summary

Tadução automática

Dit artikel beschrijft een FRET-based flow cytometrie protocol voor het kwantificeren van eiwit zelf montage in zowel S. cerevisiae en HEK293T cellen.

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