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Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
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1Bioengineering and Synthetic Microbiology Group, Instituto de Investigação e Inovação em Saude,Universidade do Porto, 2Instituto de Biologia Celular e Molecular,Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar, 4Departamento de Biologia, Faculdade de Ciências,Universidade do Porto
Capítulos
- 00:04Título
- 00:54Polymer Isolation and Removal of Contaminants
- 01:53Precipitation and Lyophilization of the Polymer
- 03:11Cyanobacterial Exoproteome Isolation
- 04:57Results: Isolation of Released Carbohydrate Polymers and Exoproteomes from Cyanobacteria Culture Media
- 06:11Conclusion
Here, protocols for the isolation of cyanobacterial released carbohydrate polymers and isolation of their exoproteomes are described. Both procedures embody key steps to obtain polymers or proteins with high purity degrees that can be used for further analysis or applications. They can also be easily adapted according to specific user needs.
