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A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
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Medicina
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JoVE Journal Medicina
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

DOI:
10.3791/59616-v

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08:04 min

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June 11, 2019

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, , , , ,
1Comprehensive Transplant Center,Northwestern University, 2Department of surgery, Feinberg School of Medicine,Northwestern University, 3Weinberg art and science college,Northwestern University

Capítulos

  • 00:04Título
  • 00:39Donor Spleen Harvest
  • 03:06Recipient Splenectomy and Spleen Graft Implantation
  • 06:28Results: Representative Syngeneic Spleen Transplantation Chimera Generation
  • 07:28Conclusion

Summary

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This protocol details the surgical steps of a mouse model of vascularized heterotopic spleen transplantation, a technically challenging model that can serve as a powerful tool in studying the fate and longevity of spleen cells, the mechanisms of distinct spleen cell populations in disease progression, and transplant immunity.

Tags

Mouse ModelVascularized Heterotopic Spleen TransplantationSpleen Cell BiologyTransplant ImmunityCongenic MiceSpleen CellsInflammatory DiseaseSurgical ProcedureDonor MouseAbdominal SurgerySpleen GraftHeparinized SalineCeliac ArteryPortal VeinSplenic Vein

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