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使用 CRISPR/Cas9 在 CAR-T 电池中敲除 GM-CSF
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Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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使用 CRISPR/Cas9 在 CAR-T 电池中敲除 GM-CSF

DOI:
10.3791/59629-v

•

07:56 min

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July 22, 2019

•

, , ,
1Mayo Clinic Medical Scientist Training Program,Mayo Clinic College of Medicine and Science, 2Department of Immunology,Mayo Clinic, 3Division of Hematology,Mayo Clinic

Capítulos

  • 00:04Título
  • 00:41T Cell Stimulation and Ex Vivo Culture
  • 02:06T Cell Transfection and Transduction
  • 03:35CART19 Cell Expansion
  • 04:48GM-CSFk/o CART19 Production
  • 06:18Results: Successful Knockout of GM-CSF in CAR-T Cells
  • 07:16Conclusion

Summary

Tadução automática

Tadução automática

在这里,我们提出一个协议,通过CRISPR/Cas9系统对CAR-T细胞进行基因编辑。

Tags

CRISPR/Cas9CAR-T CellsGenetic ModificationT-cell IsolationT-cell StimulationLentiviral VectorLentiviral Production293T CellsTransfectionVirus ConcentrationUltracentrifugation

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