Journal
/
Neurociência
/
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

DOI:
10.3791/59797-v

•

07:35 min

•

August 06, 2019

•

,
1Laboratory of RNA Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science,Charles University

Capítulos

  • 00:04Título
  • 00:34Injection
  • 02:54Cell Fixation and Staining
  • 04:07Microscopy
  • 05:09Results: Analysis of Spliceosomal snRNA Localization in Human Hela Cells
  • 07:00Conclusion

Summary

Tadução automática

Tadução automática

Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

Tags

Spliceosomal SnRNAMicroinjectionRNA LocalizationCajal BodiesHeLa CellsFluorescently Labeled RNARNA SequenceNuclear StructuresAdherent CellsMicroloaderMicroinjectorMicroscopeObjectiveCell ContactLower LimitCell Injection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/59797?article_type=v

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
check_url/pt/59797?article_type=v

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
check_url/pt/59797?article_type=v

Electrophysiological Measurements and Analysis of Nociception in Human Infants
check_url/pt/59797?article_type=v

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/pt/59797?article_type=v

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
check_url/pt/59797?article_type=v

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
check_url/pt/59797?article_type=v

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation
check_url/pt/59797?article_type=v

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
check_url/pt/59797?article_type=v

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury
check_url/pt/59797?article_type=v

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Read Article