Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Direct Gene Knock-out of Axolotl Spinal Cord…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
 

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.

Tags

Axolotl Gene Knock-out Spinal Cord Neural Stem Cells Electroporation CAS9 Protein-gRNA Complexes Molecular Mechanisms Regeneration Gene Function Developmental Effects Cell Types Spinal Cord Injury Injection Capillary Cas9 Guide RNA Ribonuclear Protein Mixture Cas9 Buffer Nuclease-free Water Minus 80 Degrees Celsius Agarose Electropor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter