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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
 

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Article DOI: 10.3791/59876-v • 07:15 min • September 12th, 2019
September 12th, 2019 •
*1, *1, 1, 1, 1,2, 3, 4, 5, 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
* These authors contributed equally

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Resumo
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This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.

Tags

Extracellular Vesicles EVs Virus-free EV Preparations EV Purification Ultracentrifugation PEG Precipitation Iodixanol Density Gradient Nanoparticle Enrichment
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