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转化、基因组编辑和拟合氮的热带大麻树帕拉斯波尼亚安德森
JoVE Journal
Genética
This content is Free Access.
JoVE Journal Genética
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
DOI:

12:22 min

August 18, 2019

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Capítulos

  • 00:04Título
  • 01:08Growth of P. andersonii Trees
  • 03:14Stable transformation of P. andersonii
  • 06:40Preparation of Rooted P. andersonii Plantlets
  • 07:31Nodulation of P. andersonii Plantlets in Pots
  • 09:48Results: Stable Transformation Procedure
  • 11:13Conclusion

Summary

Tadução automática

寄生虫树是一种快速生长的热带树,属于大麻家族(大麻),可以形成固氮根结节与rhizobium。在这里,我们描述了基于农业细菌-肿瘤介导的稳定转化和CRISPR/Cas9基于基因组编辑的P.andersonii中反向遗传分析的详细方案。

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