Medicine
É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.
Capítulos
Resumo
The combination of antibody labeling, optical clearing, and advanced light microscopy allows three-dimensional analysis of complete structures or organs. Described here is a simple method to combine immunolabeling of thick kidney slices, optical clearing with ethyl cinnamate, and confocal imaging that enables visualization and quantification of three-dimensional kidney structures.