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小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
DOI:

09:33 min

October 03, 2019

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Capítulos

  • 00:04Título
  • 01:05Early Somite Stage Embryo Dissection (Day 1)
  • 03:12Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
  • 04:36Neural Crest Single Cell Tracking
  • 05:22Quantification of Neural Crest Cell Migratory Capacity
  • 06:04Quantification of Neural Crest Cell Area and Circularity
  • 07:00Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
  • 08:43Conclusion

Summary

Tadução automática

该协议描述了从小鼠模型颅神经峰细胞的解剖和培养,主要用于研究细胞迁移。我们描述了使用的实时成像技术,并分析了速度和细胞形状的变化。

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