Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / A High-Throughput In Situ Method for Estimation…
JoVE Journal
Medicine

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicine
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
 

A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice

Article DOI: 10.3791/60095-v • 08:44 min • April 19th, 2020
April 19th, 2020 •
1, 2, 1,3, 1,3
1Centro de Investigación Príncipe Felipe (CIPF), 2Department of Physiology, Development and Neuroscience, University of Cambridge, 3Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)

Capítulos

Resumo
Automatic Translation

We present a robust, cost-effective, and flexible method for measuring changes in hepatocyte number and nuclear ploidy within fixed/cryopreserved tissue samples that does not require flow cytometry. Our approach provides a powerful sample-wide signature of liver cytology ideal for tracking the progression of liver injury and disease.

Tags

Keywords: Hepatocyte Nuclear Ploidy In Situ Method High-throughput Liver Development Liver Regeneration Chronic Liver Disease Nuclear DNA Content Tissue Sections Cryopreserved Liver Immunolabeling Ploidy Profiling
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter