Microinjection of DNA into Eyebuds in <em>Xenopus laevis</em> Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living <em>Xenopus</em> Tadpoles

Sophia Dao; Kenton Jones; Tamira Elul

doi:10.3791/60123

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Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

DOI:

10.3791/60123-v

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06:32 min

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September 04, 2019

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Sophia Dao, Kenton Jones, Tamira Elul

¹College of Osteopathic Medicine,Touro University California

Capítulos

00:04Título
00:42Loading A Microinjection Needle with DNA/DOTAP
02:15Microinjecting DNA/DOTAP into Eyebuds of One Day Old Xenopus Embryos
04:30Results: Control and Mutant Optic Axonal Arbors
05:36Conclusion

Summary

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This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

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