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Cell Type-specific Gene Expression Profiling in the Mouse Liver
 

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Article DOI: 10.3791/60242-v • 10:06 min • September 17th, 2019
September 17th, 2019 •
1, 1, 1,2
1Department of Genetics, University of Pennsylvania, 2Department of Medicine, University of Pennsylvania

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Resumo
Automatic Translation

Translating ribosome affinity purification (TRAP) enables rapid and efficient isolation of cell type-specific translating mRNA. Here, we demonstrate a method that combines hydrodynamic tail-vein injection in a mouse model of liver repopulation and TRAP to examine the expression profile of repopulating hepatocytes.

Tags

Cell Type-specific Gene Expression Profiling Mouse Liver TRAP-seq RNA Isolation Hepatocytes Gene Expression Changes Affinity Purification Liver Disease Liver Injuries New Treatments Biliary Duct Cells Kupffer Cells Endothelial Cells Magnetic Beads PBS
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