Journal
/
Neurociência
/
Pålidelig isolering af Mikrofartøjer i central nervesystemet på tværs af fem hvirveldyr grupper
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Pålidelig isolering af Mikrofartøjer i central nervesystemet på tværs af fem hvirveldyr grupper

DOI:
10.3791/60291-v

•

10:35 min

•

January 12, 2020

•

, , , ,
1Department of Anatomy, Physiology and Cell Biology,University of California Davis, 2University of Veterinary Medicine Hannover Foundation

Capítulos

  • 00:04Título
  • 00:40Central Nervous System (CNS) Tissue Dissection
  • 02:35CNS Tissue Homogenization
  • 04:20Microvessel Purification
  • 05:57Microvessel Elution and Filtration
  • 07:44Results: Representative Microvessel Protein Expression
  • 09:57Conclusion

Summary

Tadução automática

Tadução automática

Målet med denne protokol er at isolere mikrofartøjer fra flere regioner i centralnervesystemet af lissencephalic og gyrencephalic hvirveldyr.

Tags

Keywords: Microvessel IsolationCentral Nervous SystemVertebratesDissectionMeningesChoroid PlexusHomogenization

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/60291?article_type=v

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models
check_url/pt/60291?article_type=v

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
check_url/pt/60291?article_type=v

Multimodal Imaging af stamcelle implantation i det centrale nervesystem mus
check_url/pt/60291?article_type=v

Produktion af lentivirusvektorer til transduktion af celler fra det centrale nervesystem
check_url/pt/60291?article_type=v

Mærkning af enkeltceller i centralnervesystemet hos<em> Drosophila melanogaster</em
check_url/pt/60291?article_type=v

En skade Paradigm at undersøge centrale nervesystem reparation i<em> Drosophila</em
check_url/pt/60291?article_type=v

Direkte Intraventrikulær Delivery of Drugs til Rodent centrale nervesystem
check_url/pt/60291?article_type=v

In vivo Afhøring af centralnervesystemet Translatome af Polyribosome Fraktionering
check_url/pt/60291?article_type=v

In vivo optogenetic Stimulering af Rodent centralnervesystemet
check_url/pt/60291?article_type=v

Funktionel Evaluering af biologisk Neurotoksiner i Networked Kulturer af stamceller afledt centralnervesystemet neuroner

Read Article