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Journal / Neuroscience / Dissection and Isolation of Murine Glia from…
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Neuroscience

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JoVE Journal Neuroscience
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
 

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Article DOI: 10.3791/61345-v • 08:00 min • June 4th, 2020
June 4th, 2020 •
1, 1,2
1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University

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Summary
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Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

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Keywords: Murine Glia Central Nervous System Glia Heterogeneity Glia Isolation Tissue Dissection Tissue Culture Trypsin DNase I Cell Suspension Regional Differences
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