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JoVE Journal Biochemistry
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
 

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Article DOI: 10.3791/60369-v • 08:09 min • October 15th, 2019
October 15th, 2019 •
1, 1,2
1Department of Biological Sciences, Smith College, 2Center for Microscopy and Imaging, Smith College

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The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.

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Dynein Kinesin DNA Origami Nanostructures Single Molecule Observation Motor Proteins Biophysical Mechanisms Cargo Transport Cytoskeletal Motor Proteins Microtubule-based Motors Dynein Kinesin Actin-based Motor Myosin Protein Systems Motor Protein Growth And Expression Galactose Promoter Yeast Strain Yeast-peptone-dextrose Culture Plate Incubation Optical Density Centrifugation Cell Slurry
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