Journal
/
Biologia do Desenvolvimento
/
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

DOI:
10.3791/60379-v

•

08:30 min

•

March 04, 2020

•

, , , , ,
1Department of Pathology,Yale University School of Medicine, 2Stem Cell Center,Yale University School of Medicine

Capítulos

  • 00:05Introduction
  • 00:53Stem Cell Maintenance
  • 03:03Dissociation of the hESCs for Organoid Culture
  • 04:27Generation of Organoids
  • 06:06Results: Overview of Organoid Growth Conditions and Morphology
  • 07:37Conclusion

Summary

Tadução automática

Tadução automática

This protocol was generated as a means to produce brain organoids in a simplified, low cost manner without exogenous growth factors or basement membrane matrix while still maintaining the diversity of brain cell types and many features of cellular organization.

Tags

Brain OrganoidsHuman Embryonic Stem CellsNeurodevelopmental DisordersToxic InsultsBrain CancerReproducibleSimple WorkflowCost-effectiveMatrixH9 HESCsMTeSR1 MediaLow Oxygen IncubatorProtease SolutionPassaging

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/60379?article_type=v

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
check_url/pt/60379?article_type=v

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
check_url/pt/60379?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/pt/60379?article_type=v

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
check_url/pt/60379?article_type=v

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
check_url/pt/60379?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/pt/60379?article_type=v

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
check_url/pt/60379?article_type=v

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
check_url/pt/60379?article_type=v

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
check_url/pt/60379?article_type=v

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Read Article