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生物应用近红外发光金纳米簇的合成

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

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生物应用近红外发光金纳米簇的合成

DOI:

10.3791/60388-v

•

09:11 min

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March 22, 2020

•

Goutam Pramanik, Alena Keprova, Jan Valenta, Vaclav Bocan, Klaudia Kvaková⁴, Lenka Libusova, Petr Cigler

¹Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, ²Department of Chemical Physics and Optics, Faculty of Mathematics and Physics,Charles University, ³Department of Cell Biology, Faculty of Science,Charles University, ⁴First Faculty of Medicine,Charles University

Capítulos

00:05Introduction
01:10Preparation of Near-infrared Emitting AuNCs
02:13Conjugation of TPP on the Surface of 1
02:55AuNC Internalization into HeLa Cells
03:55Flow Cytometry Analysis
05:26CLSM Imaging of Live HeLa Cells Labeled with 2
07:15Results: Gold Nanoclusters inside HeLa Cells
08:23Conclusion

Summary

Tadução automática

介绍了一种可靠且易于重现的方法，用于制备可功能的近红外发光金纳米簇，并通过流细胞学和共聚焦激光扫描显微镜直接检测HeLa细胞内部。

Tags

Keywords: Gold Nanoclusters Near-infrared Emitting Photoluminescent Thiol-functionalized Ligand Amine-functionalized Ligand Biosensing Bio-imaging Ultrafiltration Polyethylene Glycol EDC HCL Cell Culture

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