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Biologia
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Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
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Biologia
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JoVE Journal Biologia
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

DOI:
10.3791/60451-v

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08:25 min

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October 31, 2019

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, , , ,
1Institute of Molecular Plant Sciences, School of Biological Sciences,University of Edinburgh, 2Centre for Synthetic and Systems Biology,University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences,University of Edinburgh

Capítulos

  • 00:04Título
  • 01:11Construction of Level T Assemblies
  • 01:43Generation of Mutants by Conjugation
  • 06:46Results: S. elongatus UTEX 2973 and Synechocystis PCC 6803
  • 08:03Conclusion

Summary

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Tadução automática

Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.

Tags

Genetic ModificationCyanobacteriaConjugationCyanoGateModular CloningSynthetic BiologyRenewable BiotechnologyTriparental MatingTransformationSynechocystisSynechococcusE. ColiHelper StrainPlasmids

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