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定量荧光显微镜为基础的单脂体测定，用于检测单个脂质体之间的成分不均匀性

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Bioengenharia

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JoVE Journal Bioengenharia

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

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定量荧光显微镜为基础的单脂体测定，用于检测单个脂质体之间的成分不均匀性

DOI:

10.3791/60538-v

•

09:12 min

•

December 13, 2019

•

Rasmus Münter³, Thomas Lars Andresen^2,3, Jannik Bruun Larsen^2,3

¹Center for Nanomedicine and Theranostics,Technical University of Denmark, ²Center for Intestinal Absorption and Transport of Biopharmaceuticals,Technical University of Denmark, ³Department of Health Technology,Technical University of Denmark

Capítulos

00:04Título
00:58Liposome Preparation
02:14Surface Preparation of Imaging Chamber
02:57Liposome Immobilization
03:42Image Acquisition and Data Analysis
05:21Liposome Size Calibration
06:42Results: Liposome Surface Immobilization and Compositional Inhomogeneity
08:05Conclusion

Summary

Tadução automática

该协议描述了脂体的制造，以及如何用荧光显微镜将这些脂体固定在表面上，并单独以大规模平行的方式成像。这允许定量种群单脂质体之间的大小和组成不均匀性。

Tags

Keywords: Single Liposome Liposome Composition Liposome Inhomogeneity Fluorescence Microscopy Lipid Labeling Lipid Mixing Freeze-thaw Extrusion BSA Coating Streptavidin Single-molecule Detection

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