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JoVE Journal Developmental Biology
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
 

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Article DOI: 10.3791/60551-v • 10:00 min • November 28th, 2019
November 28th, 2019 •
1, 1, 2, 3, 1, 4, 1
1Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica, Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM

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This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

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Keywords: In Vitro Culture Epithelial Cells Human Amniotic Membrane Anatomical Regions Umbilical Amnion Placental Amnion Reflected Region Dissection Saline Wash Cell Isolation
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