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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

DOI:
10.3791/60551-v

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10:00 min

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November 28, 2019

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, , , , , ,
1Departamento de Fisiología y Desarrollo Celular,Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT,Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica,Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM

Chapters

  • 00:04Title
  • 00:43Mechanical Amniotic Membrane Separation
  • 02:40Membrane Washing
  • 03:36Enzymatic Amniotic Membrane Digestion
  • 06:21Human Amniotic Epithelial Cells (HAEC) Isolation and Culture
  • 07:31Results: Representative HEAC Morphologic and Phenotypic Analyses
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

Tags

In Vitro CultureEpithelial CellsHuman Amniotic MembraneAnatomical RegionsUmbilical AmnionPlacental AmnionReflected RegionDissectionSaline WashCell Isolation

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