JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
ヒト羊膜の異なる解剖学的領域からの上皮細胞のインビトロ培養
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Please note that all translations are automatically generated.
Click here for the English version.
ヒト羊膜の異なる解剖学的領域からの上皮細胞のインビトロ培養
DOI:
10.3791/60551-v
•
10:00 min
•
November 28, 2019
•
Daniela Avila-González
,
Guadalupe García-López
,
Néstor E. Díaz-Martínez
,
Héctor Flores-Herrera
,
Anayansi Molina-Hernández
,
Wendy Portillo
,
Néstor F. Díaz
1
Departamento de Fisiología y Desarrollo Celular
,
Instituto Nacional de Perinatología
,
2
Biotecnología Médica y Farmacéutica CONACYT
,
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ)
,
3
Departamento de Inmunobioquímica
,
Instituto Nacional de Perinatología
,
4
Instituto de Neurobiología, UNAM
Chapters
00:04
Title
00:43
Mechanical Amniotic Membrane Separation
02:40
Membrane Washing
03:36
Enzymatic Amniotic Membrane Digestion
06:21
Human Amniotic Epithelial Cells (HAEC) Isolation and Culture
07:31
Results: Representative HEAC Morphologic and Phenotypic Analyses
09:12
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルは、ヒト羊膜の異なる解剖学的領域からの上皮細胞の単離を記述し、臨床および生理学的病理学モデルにおける可能な適用のための異質性および機能的特性を決定する。
Tags
In Vitro Culture
Epithelial Cells
Human Amniotic Membrane
Anatomical Regions
Umbilical Amnion
Placental Amnion
Reflected Region
Dissection
Saline Wash
Cell Isolation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
ウズラ胚の卵黄嚢の膜からの一次内胚葉上皮細胞培養
開発<em>インビトロ</em>アッセイは、間葉細胞受けた上皮間葉移行の収縮機能を評価します
再現性の高い小分子ベースの分化プロトコルを使用したヒト多能性幹細胞から心筋細胞の大規模生産
トランスポゾンのシステムを用いたマウスの羊水細胞から多能性幹細胞の作製
マウス子宮内膜上皮と間質細胞の単離のために<em>インビトロ</em>脱落膜化
ひと多能性幹細胞からプリミティブかつ決定的な造血前駆細胞の分化を監督
主な羊水および膜 Xeno 無料条件で多能性細胞をリプログラミング
人間の胚または誘導多能性幹細胞からの網膜色素上皮細胞の急速な監督の分化
造血の発達における大動脈生殖巣中植文化システムのアプリケーション
高齢者人間ブルッフ膜の
Ex Vivo
モデルの網膜色素上皮細胞の培養
Read Article