Journal
/
Neurociência
/
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

DOI:
10.3791/60554-v

•

07:04 min

•

April 12, 2020

•

,
1Davee Department of Neurology,Northwestern University Feinberg School of Medicine Chicago, 2Department of Cell and Molecular Biology,Northwestern University Feinberg School of Medicine Chicago

Capítulos

  • 00:05Introduction
  • 00:53Cerebellum Dissection and Cell Suspension Preparation
  • 02:34Immunolabeling of Stem Cells
  • 03:44Magnetic Column Preparation and Cell Sorting
  • 04:22Passaging of Neurospheres and Differentiation
  • 05:27Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
  • 06:15Conclusion

Summary

Tadução automática

Tadução automática

Demonstrated here is an efficient and cost-effective method to purify, culture, and differentiate white matter stem cells from postnatal mouse cerebellum.

Tags

Prominin-1Stem CellsCerebellumPostnatalFACSPapainDNAseCentrifugationCell Strainer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/60554?article_type=v

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
check_url/pt/60554?article_type=v

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
check_url/pt/60554?article_type=v

Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
check_url/pt/60554?article_type=v

Purification of Mouse Brain Vessels
check_url/pt/60554?article_type=v

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
check_url/pt/60554?article_type=v

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains
check_url/pt/60554?article_type=v

Isolation and Culture of Embryonic Mouse Neural Stem Cells
check_url/pt/60554?article_type=v

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
check_url/pt/60554?article_type=v

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
check_url/pt/60554?article_type=v

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

Read Article