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JoVE Journal Biochemistry
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
 

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Article DOI: 10.3791/60607-v • 04:45 min • February 10th, 2022
February 10th, 2022 •
*1, *2, *1,3,4, 1,3,4, 1,3
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Chemistry, University of South Florida, 3Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 4Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.

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Keywords: Microscale Thermophoresis Protein-lipid Interactions Vam7 Phosphatidic Acid Disassociation Constant (KD) Biochemical Interactions NTA-Atto 647 Dye Thermophoretic Traces 1:1 Titration DiC8 PA Pharmaceutical Development
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