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用于阴道内艾滋病毒暴露和艾滋病毒感染的人性化 NOG 小鼠

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

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用于阴道内艾滋病毒暴露和艾滋病毒感染的人性化 NOG 小鼠

DOI:

10.3791/60723-v

•

08:15 min

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January 31, 2020

•

Anna H. F. Andersen², Stine S. F. Nielsen², Rikke Olesen², Katharina Mack, Frederik Dagnæs-Hansen, Niels Uldbjerg⁴, Lars Østergaard², Ole S. Søgaard², Paul W. Denton^2,5, Martin Tolstrup²

¹Department of Clinical Medicine,Aarhus University, ²Department of Infectious Diseases,Aarhus University Hospital, ³Department of Biomedicine,Aarhus University, ⁴Department of Gynecology and Obstetrics,Aarhus University Hospital, ⁵Department of Biology,University of Nebraska at Omaha

Capítulos

00:04Título
00:50Cord Blood CCR5Δ32 Genetic Variant Screening
01:50Intravaginal HIV Exposure
03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
05:42Results: Representative HIV infection and Treatment DATA
07:13Conclusion

Summary

Tadução automática

我们开发了一种基于干细胞移植、阴道内人体免疫缺陷病毒暴露和滴数字PCR RNA的人性化和人体免疫缺陷病毒感染NOG小鼠模型的生成和评估方案量化。

Tags

Keywords: Humanized NOG Mice Intravaginal HIV Exposure HIV Infection Preclinical HIV Research Viral Strains HIV Interventions CCR5 Variant Viral RNA Quantification Cord Blood Screening CCR5 Delta 32 HIV Exposure Protocol

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