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Immunology and Infection

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JoVE Journal Immunology and Infection
An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
 

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Article DOI: 10.3791/60790-v • 07:32 min • December 3rd, 2020
December 3rd, 2020 •
*1, *1, 1, 1, 1, 2, *1, *1, *1
1Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, 2Norwich Medical School, University of East Anglia, Norwich Research Park
* These authors contributed equally

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Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.

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Intravital Microscopy Intestinal Permeability Epithelial Cell Shedding Real-time Visualization Single-cell Resolution Dynamic Processes Tracer Experiments Para And Transcellular Permeability Visualizing Cellular Processes Surgical Preparation Steps Confocal Laser Scanning Microscope Image Acquisition Software Image Resolution Acquisition Mode Objective Sequential Acquisition Setting
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