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JoVE Journal Immunology and Infection
Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
 

Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry

Article DOI: 10.3791/60810-v • 07:20 min • May 19th, 2020
May 19th, 2020 •
1,2, 3, 2,4, 2,4, 2, 5,6
1Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine, University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, 4Department of Immunology, Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine, University of Reims Champagne-Ardenne

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The aim of this method is to determine the CR1 density in the erythrocytes of any subject by comparing with three subjects whose erythrocyte CR1 density is known. The method uses flow cytometry after immunostaining of the subjects' erythrocytes by an anti-CR1 monoclonal antibody coupled to an amplified system using phycoerythrin (PE).

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Erythrocyte Complement Receptor 1 Flow Cytometry Antigenic Sites Cellular Receptor Robust Results Low Density Receptors CR1 Erythrocyte Expression Reduction Alzheimer's Systemic Lupus Erythematosus AIDS Malaria Cell Receptor Density Analysis Fluorescence Microscope Cytometric Specialist Immunostaining Density Quantification Analysis Parameters Sodium EDTA Anticoagulated Whole Blood PBS-BSA Solution
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