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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
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Bioquímica
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JoVE Journal Bioquímica
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

DOI:
10.3791/60813-v

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08:02 min

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March 12, 2020

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, , , ,
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, 2Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Capítulos

  • 00:05Introduction
  • 00:49Colon Sample Preparation
  • 03:12Immunohistochemistry
  • 06:07Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
  • 07:20Conclusion

Summary

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Tadução automática

The protocol presents a complete validated TNBS-induced murine model of Crohn's disease and methods for visualization of estrogen receptors by immunohistochemistry using immunofluorescence of formalin-fixed colon sections embedded in paraffin.

Tags

Estrogen ReceptorImmunofluorescenceTNBS-induced Crohn’s DiseaseMouse ColonParaffin EmbeddingMicrotome SectioningDeparaffinization

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