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生产自体富血小板血浆以促进体外人成纤维细胞扩增
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JoVE Journal Bioengenharia
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
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生产自体富血小板血浆以促进体外人成纤维细胞扩增

DOI:
10.3791/60816-v

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08:34 min

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February 24, 2021

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, ,
1Department of Plastic, Reconstructive and Aesthetic Surgery, Geneva University Hospitals, Faculty of Medicine,Geneva University, 2Regen Lab SA

Capítulos

  • 00:04Introduction
  • 01:01Preparation of Platelet‐Rich Plasma (PRP)
  • 02:02Isolation and Culture of Autologous Fibroblasts
  • 06:13Results: Standardized 1.5X Leukocyte Poor PRP Boosts Cell Proliferation
  • 07:26Conclusion

Summary

Tadução automática

Tadução automática

该协议提出了一种产生PRP以促进100%自体成纤维细胞培养系统中细胞体外扩增的装置。

Tags

Autologous Platelet-rich PlasmaFibroblast ExpansionCell CultureCell TherapyCollagenase/dispaseCentrifugationPRP Preparation

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