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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
 

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Article DOI: 10.3791/60945-v • 10:45 min • June 20th, 2020
June 20th, 2020 •
1, 1, 2, 1, 3
1SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

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The overall goal of the protocol is to prepare over one million ordered, uniform, stable, and biocompatible femtoliter droplets on a 1 cm2 planar substrate that can be used for cell-free protein synthesis.

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Keywords: Femtoliter Droplet Array Single DNA Molecules Parallel Protein Synthesis Enzyme Activity Measurement Microfabrication Photolithography CYTOP Polymer Spin Coating Reactive-ion Etching
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