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JoVE Journal Biochemistry
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
 

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Article DOI: 10.3791/60950-v • 07:49 min • June 8th, 2020
June 8th, 2020 •
1, *1, *1,2, 1
1School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College
* These authors contributed equally

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Conventional BODIPY conjugates can be used for live-cell single-molecule localization microscopy (SMLM) through exploitation of their transiently forming, red-shifted ground state dimers. We present an optimized SMLM protocol to track and resolve subcellular neutral lipids and fatty acids in living mammalian and yeast cells at the nanoscopic length scale.

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Keywords: BODIPY Conjugates Super-resolution Microscopy Single-molecule Tracking Live-cell Imaging Lipid Droplets Fatty Acid Yeast Cells Mammalian Cells EMCCD Camera Laser Power Microscope Optimization
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