/
/
Samtidig kvantificering af udvalgte kynureniner analyseret af flydende kromatografi-massespektrometri i medium indsamlet fra kræftcellekulturer
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:573-Nitrotyrosine (3NT), Kynurenine (Kyn), 3-Hydroxykynurenine (3HKyn), 3-Hydroxyanthranilic Acid (3HAA), Xanthurenic Acid (XA) and Charcoal Treated Culture Medium Preparation
- 01:58Calibration Solution Preparation
- 03:42Liquid Chromatography-Mass Spectrometry (LC-MS) System Setu
- 04:20Calibration Curve Construction
- 04:55Sample Collection
- 05:33Sample Preparation for LC-MS Analysis
- 06:30Protein Content Assessment and Data Normalization
- 08:12Results: Representative LC-MS Data of Selected KYNURENINES Determination
- 09:57Conclusion
Beskrevet her er en protokol til bestemmelse af fire forskellige tryptofan metabolitter genereret i kynureninvejen (kynurenin, 3-hydroxykynurenin, xanthurensyre, 3-hydroxyanthranilic syre) i mediet indsamlet fra kræftcellekulturer analyseret af flydende kromatografi kombineret med en enkelt firdoblet massespektrometri.
