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JoVE Journal Bioengineering
Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
 

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures

Article DOI: 10.3791/61031-v • 10:38 min • May 9th, 2020
May 9th, 2020 •
1, 1, 1
1Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin

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Described here is a protocol for the determination of four different tryptophan metabolites generated in the kynurenine pathway (kynurenine, 3-hydroxykynurenine, xanthurenic acid, 3-hydroxyanthranilic acid) in the medium collected from cancer cell cultures analyzed by liquid chromatography coupled with a single quadrupole mass spectrometry.

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Liquid Chromatography-mass Spectrometry Kynurenines Cancer Cell Cultures Immune Response Regulation Tryptophan Metabolites Sample Preparation Data Acquisition Data Interpretation Stock Solutions Reagents Charcoal-treated Culture Medium
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