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Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry

Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry

DOI:
10.3791/61048-v

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07:02 min

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April 29, 2020

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,
1Microbiology and Cell Science Department,University of Florida

Capítulos

  • 00:04Introduction
  • 00:37Correlating Optical Density and Bacterial Concentration
  • 02:14VLP Attachment
  • 03:29Antibody Staining
  • 05:19Results: Quantifying VLP Binding
  • 06:15Conclusion

Summary

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The goal of this protocol is to quantify binding of the eukaryotic pathogen human norovirus to bacteria. After performing an initial virus-bacterium attachment assay, flow cytometry is used to detect virally-bound bacteria within the population.

Tags

Virus-bacterial InteractionsHuman NorovirusVirus-like ParticlesFlow CytometryEnterobacter CloacaeBacterial QuantificationSerial DilutionsOptical DensityColony Forming Units

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