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P. 阿鲁吉诺萨 气液界面中支气皮细胞和巨噬细胞的感染三维共培养，用于抗感染的临床前评估
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
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P. 阿鲁吉诺萨 气液界面中支气皮细胞和巨噬细胞的感染三维共培养，用于抗感染的临床前评估

DOI:
10.3791/61069-v

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10:26 min

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June 15, 2020

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, , , , , , , ,
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital

Capítulos

  • 00:04Introduction
  • 01:26Growth and Differentiation of Cells in Permeable Support Inserts
  • 05:29Infection by P. aeruginosa
  • 07:00Measurement of Bacterial Proliferation via Colony-forming Units (CFU)
  • 08:00Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa
  • 09:48Conclusion

Summary

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我们描述了一个协议，一个三维共培养模型的感染气道，使用CFBE41o- 细胞，THP-1 巨噬细胞，和伪多莫纳斯aeruginosa，建立在空气-液体接口。该模型提供了一个新的平台，同时测试抗生素疗效，上皮屏障功能和炎症标记。

Tags

Keywords: P. Aeruginosa3D Co-cultureBronchial Epithelial CellsMacrophagesAir-liquid InterfaceAnti-infectivesBiofilmsHost CellsEpithelial BarriersLung InfectionAerosol AdministrationHuman CellsInfection ResearchCell CultivationCFBE41 O Minus CellsCell SeedingPermeable Supports

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