Journal
/
Biologia
/
质谱分析，用于识别 EYFP 标记 CENP-A （EYFP-CENP-A） 的泛化
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
Please note that all translations are automatically generated. Click here for the English version.

质谱分析，用于识别 EYFP 标记 CENP-A （EYFP-CENP-A） 的泛化

DOI:
10.3791/61138-v

•

09:02 min

•

June 10, 2020

•

, , , , , , ,
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center,Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine,Medical School of Nanjing University, 3Greehey Children’s Cancer Institute, Department of Molecular Medicine,UT Health Science Center San Antonio

Capítulos

  • 00:04Introduction
  • 00:44In Vivo Ubiquitylation Assays Using pQCXIP-EYFP-CENP-A
  • 02:19Mass Spectrometry to Identify the Ubiquitylation Site of the EYFP-CENP-A K124R Mutant
  • 04:55Data Analysis
  • 06:22Results: EYFP-CENP-A K124 Mutant is Ubiquitylated and Interacts with HJURP
  • 08:12Conclusion

Summary

Tadução automática

Tadução automática

CENP-A泛化是CENP-A在中心区沉积的重要要求，通过细胞分裂之间的二化遗传，对细胞的生存能力不可或缺。在这里，我们描述质谱分析，以确定EYFP标记CENP-A（EYFP-CENP-A）蛋白的泛化。

Tags

Mass SpectrometryUbiquitylationEYFP-CENP-AProtein IdentificationImmunoprecipitationGFP AntibodyLysate PreparationGel ElectrophoresisCoomassie Blue StainingProtein DigestionMass Spectrometry Analysis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

MALDI样品制备：超薄层的方法

大蛋白复合物结构质谱分析

T波离子迁移质谱蛋白复合物分析的基本实验程序

在大气压力质谱直接分析单细胞

酰胺氢/氘交换和MALDI - TOF质谱PAK2激活的质谱分析

利用同位素标记的质谱剖析巯基氧化还原蛋白质

捕获复合质谱 - 一个强大的工具来确定新型C-二GMP效应蛋白

siRNA的筛选识别泛素和泛素样系统压力表生物学途径在培养的哺乳动物细胞

从富集组织及消化细胞外基质蛋白的成肽质谱分析

单探头的应用：根据环境条件质谱成像和单细胞分析

Read Article