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Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells
DOI:

09:15 min

May 11, 2020

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Capítulos

  • 00:04Introduction
  • 00:42Cigarette Smoking Extract (CSE) Preparation
  • 01:39Pseudomonas Culture
  • 02:16Cell Culture and CSE Treatment
  • 03:33Bacterial Infection and Gentamycin Treatment
  • 04:24Determination of Bacterial Concentration Using the Drop Plate Method
  • 04:55RT-qPCR Detection of Bacterial 16S rRNA
  • 07:00Results: CSE-Treatment Increases Bacterial Load
  • 08:23Conclusion

Summary

Tadução automática

Described here is a protocol to study how cigarette smoke extract affects bacterial colonization in lung epithelial cells.

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