Journal
/
Bioquímica
/
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

DOI:
10.3791/61229-v

•

06:10 min

•

June 18, 2020

•

, , ,
1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Capítulos

  • 00:04Introduction
  • 00:46Cell Preparation
  • 01:33Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction
  • 02:46Cysteine/Cystine (CyS/CySS) Balance Acquisition
  • 04:01Results: Representative Cellular Oxidation Evaluation
  • 05:29Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

Tags

Subcellular CompartmentRedox-sensitive GFPOxidative StressRedox StateRatiometricNon-disruptiveTransductionFlow CytometryHydrogen Peroxide

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61229?article_type=v

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
check_url/pt/61229?article_type=v

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
check_url/pt/61229?article_type=v

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
check_url/pt/61229?article_type=v

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
check_url/pt/61229?article_type=v

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
check_url/pt/61229?article_type=v

Extraction and Analysis of Taiwanese Green Propolis
check_url/pt/61229?article_type=v

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
check_url/pt/61229?article_type=v

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
check_url/pt/61229?article_type=v

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
check_url/pt/61229?article_type=v

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Read Article