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监测三D培养中的癌细胞入侵和T细胞毒性

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Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

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监测三D培养中的癌细胞入侵和T细胞毒性

DOI:

10.3791/61392-v

•

07:44 min

•

June 23, 2020

•

Yuan-Na Lin, Apsra Nasir, Sharon Camacho, Deborah L. Berry, Marcel O. Schmidt, Gray W. Pearson, Anna T. Riegel, Anton Wellstein

¹Lombardi Comprehensive Cancer Center, Department of Oncology,Georgetown University

Capítulos

00:04Introduction
00:37Spheroid Generation
01:43T Cell Co-Culture
02:393D Co-Culture Embedding
03:41Immunofluorescence StainingImmunofluorescence Staining
05:43Results: Representative Cancer Cell Invasion Monitoring
07:18Conclusion

Summary

Tadução automática

该方法同时评价了三D球类测定和T细胞毒性中的癌细胞入侵。球体在无脚手架的阿加罗斯多微井铸件中生成。I型胶原蛋白基质中的共同培养和嵌入在同一设备中进行，允许监测癌细胞入侵和T细胞中角细胞毒性。

Tags

3D Culture Cancer Cell Invasion T-cell Cytotoxicity Spheroid Co-culture Agarose Mold Tumor Cell Suspension T-cell Co-culture Type I Collagen 3D Co-culture Mechanistic Insights Immune Cell-mediated Tumor Cell Cytotoxicity Invasive Behavior

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