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Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
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Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

DOI:
10.3791/61400-v

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10:12 min

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July 14, 2020

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, , ,
1Institute for Hygiene and Microbiology,University of Würzburg, 2Department of Biological Sciences,University of Alabama

Capítulos

  • 00:05Introduction
  • 00:42IMR90-4 Cell Culture Maintenance
  • 02:15Differentiation of Brain Endothelial Cells from Human iPSCs
  • 05:57Transendothelial Electrical Resistance (TEER) as a Measure of Barrier Tightness
  • 06:35Preparation of Bacteria and Infection of iPSC-BECs
  • 08:22Results: Upregulation of Cytokines by iPSC-BECs upon Infection with Neisseria meningitidis
  • 09:16Conclusion

Summary

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The protocol described here highlights the major steps in the differentiating induced pluripotent stem-cell derived brain-like endothelial cells, the preparation of Neisseria meningitidis for infection, and sample collection for other molecular analyses.

Tags

IPSC-derived Brain Endothelial CellsBlood-brain BarrierNeisseria MeningitidisHost-pathogen InteractionsCell CultureCell PassagingCell Counting

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