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JoVE Journal Bioengineering
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
 

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Article DOI: 10.3791/61404-v • 09:55 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 1, 1, 1,2
1Physiopathogénèse et traitement des maladies du foie, Université Paris-Saclay, Inserm U1193, 2ESPCI Paris, Université PSL
* These authors contributed equally

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Three-dimensional (3D) cellular systems are relevant models for investigating organogenesis. A hydrogel-based method for biliary cysts production and their characterization is proposed. This protocol unravels the barriers of 3D characterization, with a straightforward and reliable method to assess cyst formation efficiency, sizes, and to test their functionality.

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Biliary Cysts Quantitative Characterization Functional Epithelial Cysts Polarized Biliary Epithelial Cysts Evaluation Efficiency Size Therapeutic Options Drug Studies Therapeutic Targets Disease Mechanisms Hydrogel Solution Cholangiocyte Complete Medium Chamber Slide Bioengineering Tubular Structures
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