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Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
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JoVE Journal Pesquisa do Câncer
Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

DOI:
10.3791/61449-v

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08:25 min

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September 09, 2020

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, ,
1Cancer Genomics and BioComputing of Complex Diseases laboratory, The Azrieli Faculty of Medicine,Bar-Ilan University, 2The Data Science Institute,Bar-Ilan University, 3The Dangoor Centre For Personalized Medicine,Bar-Ilan University

Capítulos

  • 00:04Introduction
  • 00:32Circulating Cell-Free DNA (cfDNA) Purification
  • 03:18DNA Fragment Size Analysis
  • 06:18Results: Representative Glioma Patient cfDNA Bioanalyses
  • 07:59Conclusion

Summary

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In this paper we present a detailed protocol for non-invasive liquid biopsy technique, including blood collection, plasma and buffy coat separation, cfDNA and germline DNA extraction, quantification of cfDNA or germline DNA, and cfDNA fragment enrichment analysis.

Tags

Cell-free DNALiquid BiopsyTumor MutationsTumor FusionsNon-invasiveCancer Disease ProgressionPlasma SampleProteinase KLysis BufferBinding BufferSilica Membrane ColumnWash BufferEthanolElution BufferDNA Fragment Analysis

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