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癌症患者血浆样本中无细胞DNA检测

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JoVE Journal Pesquisa do Câncer

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

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癌症患者血浆样本中无细胞DNA检测

DOI:

10.3791/61449-v

•

08:25 min

•

September 09, 2020

•

Vikrant Palande, Dorith Raviv Shay, MILANA Frenkel-Morgenstern^2,3

¹Cancer Genomics and BioComputing of Complex Diseases laboratory, The Azrieli Faculty of Medicine,Bar-Ilan University, ²The Data Science Institute,Bar-Ilan University, ³The Dangoor Centre For Personalized Medicine,Bar-Ilan University

Capítulos

00:04Introduction
00:32Circulating Cell-Free DNA (cfDNA) Purification
03:18DNA Fragment Size Analysis
06:18Results: Representative Glioma Patient cfDNA Bioanalyses
07:59Conclusion

Summary

Tadução automática

本文提出了非侵入性液体活检技术的详细方案，包括采血、血浆和缓冲涂层分离、cfDNA和生殖系DNA提取、cfDNA或生殖系DNA的量化以及cfDNA片段富集分析。

Tags

Keywords: Cell-free DNA Liquid Biopsy Tumor Mutations Tumor Fusions Non-invasive Cancer Disease Progression Plasma Sample Proteinase K Lysis Buffer Binding Buffer Silica Membrane Column Wash Buffer Ethanol Elution Buffer DNA Fragment Analysis

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