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Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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Capítulos
- 00:05Introduction
- 00:48Microscope Slide Preparation
- 02:06Single Particle Darkfield Microscopy (DFM) Tracking
- 02:53Data Acquisition
- 04:21Red/Green Values
- 04:59Data Analysis
- 05:30Mean Square Displacement (MSD), Multi-Particle, and Time Analyses
- 06:33Results: Representative Single Nanoparticle DFM of AuNR Diffusion Dynamics
- 08:39Conclusion
Here, we show the use of traditional dark-field microscopy to monitor the dynamics of gold nanorods (AuNRs) on cell membrane. The location and orientation of single AuNRs are detected using ImageJ and MATLAB, and the diffusive states of AuNRs are characterized by single particle tracking analysis.
