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Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
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Neurociência
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JoVE Journal Neurociência
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

DOI:
10.3791/61629-v

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08:04 min

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November 19, 2020

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, ,
1Center for Genomic Medicine,Massachusetts General Hospital, 2Department of Psychiatry,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4Department of Psychiatry,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital

Capítulos

  • 00:04Introduction
  • 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
  • 01:46iPSC-Derived BMEC Subculture and Purification
  • 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
  • 04:09Efflux Transporter Activity and Analysis
  • 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
  • 07:18Conclusion

Summary

Tadução automática

Tadução automática

This protocol details an adapted method to derive, expand, and cryopreserve brain microvascular endothelial cells obtained by differentiating human induced pluripotent stem cells, and to study blood brain barrier properties in an ex vivo model.

Tags

Brain Microvascular Endothelial CellsHuman Induced Pluripotent Stem CellsBlood-brain BarrierBMEC DerivationBMEC ExpansionBMEC CryopreservationBMEC CharacterizationTEER AnalysisEfflux Transporter ActivityDisease Modeling

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