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JoVE Journal Neuroscience
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
 

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Article DOI: 10.3791/61629-v • 08:04 min • November 19th, 2020
November 19th, 2020 •
1,2,3, 1,2,3,4, 1,3,4,5
1Center for Genomic Medicine, Massachusetts General Hospital, 2Department of Psychiatry, Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, 4Department of Psychiatry, Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program, McLean Hospital

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Summary
Automatic Translation

This protocol details an adapted method to derive, expand, and cryopreserve brain microvascular endothelial cells obtained by differentiating human induced pluripotent stem cells, and to study blood brain barrier properties in an ex vivo model.

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Keywords: Brain Microvascular Endothelial Cells Human Induced Pluripotent Stem Cells Blood-brain Barrier BMEC Derivation BMEC Expansion BMEC Cryopreservation BMEC Characterization TEER Analysis Efflux Transporter Activity Disease Modeling
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