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JoVE Journal Neuroscience
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
 

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Article DOI: 10.3791/50321-v • 10:49 min • April 14th, 2013
April 14th, 2013 •
1,2, 1, 1, 1,3
1Department of Neuroscience, The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology, The University of Connecticut Health Center, 3Stem Cell Institute, The University of Connecticut Health Center

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Summary
Automatic Translation

This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

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Keywords: Human Pluripotent Stem Cells Telencephalic Glutamatergic Neurons Neural Induction Neuroepithelial Cells Telencephalic Precursors Dorsal Telencephalic Progenitors Glutamatergic Neurons
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