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Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
 

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Article DOI: 10.3791/61713-v • 08:29 min • September 9th, 2020
September 9th, 2020 •
*1, *1, 1
1Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF - MC), Goethe-Universität
* These authors contributed equally

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Light sheet-based fluorescence microscopy is the most valuable tool in developmental biology. A major issue in comparative studies is ambient variance. Our protocol describes an experimental framework for simultaneous live imaging of multiple specimens and, therefore, addresses this issue pro-actively.

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Simultaneous Live Imaging Multiple Insect Embryos Sample Chamber-based Light Sheet Fluorescence Microscopes High Content High Throughput Live Imaging Assays Ambient Variance Cobweb Holder Stacking Embryos Increasing Throughput Mounting Embryos Finesse Explanatory Video Conduct Gestures Calibration Reliquify Agarose Aliquot Dry Block Heater Mixer Fluorescent Microsphere Solution Slotted Hole Solidify Agarose Sample Chamber Autoclaved Tap Water
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