/

/

纤维蛋白富集和 tPA 敏感的光血栓形成卒中模型

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model

Please note that all translations are automatically generated. Click here for the English version.

纤维蛋白富集和 tPA 敏感的光血栓形成卒中模型

DOI:

10.3791/61740-v

•

09:42 min

•

June 04, 2021

•

Yi-Min Kuo, Yu-Yo Sun, Chia-Yi Kuan

¹Department of Anesthesiology, Taipei Veterans General Hospital,National Yang-Ming University School of Medicine, ²Department of Neuroscience, Center for Brain Immunology and Glia (BIG),University of Virginia School of Medicine

Capítulos

00:04Introduction
01:19Ligation of the Ipsilateral Common Carotid Artery
02:35Skull Thinning Above the Middle Cranial Artery Branch and Photoactivation
04:44Intravital Imaging and tPA Administration
06:34Results: Immunofluorescence Labeling, Intravital Imaging, and the Effects of tPA Treatment on CBF Recovery and Infarct Size
08:50Conclusion

Summary

Tadução automática

传统的光血栓中风（PTS）模型主要诱导对组织纤溶酶原激活剂（tPA）溶解治疗具有高耐药性的致密血小板聚集体。本文通过共注射凝血酶和光敏染料进行光活化，引入了改良的小鼠PTS模型。凝血酶增强的 PTS 模型产生混合血小板：纤维蛋白凝块，并且对 tPA 溶栓高度敏感。

Tags

Photothrombotic Stroke Model Fibrin-enriched TPA-sensitive Thrombolytic Therapy Blood Clot Acute Stroke Patient Preclinical Stroke Research Thrombolytic Therapies

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

逆行神经，大港，并用荧光染料的神经根装载

来自祖，人脑少突胶质细胞培养系统

星形细胞转运电流派生谷氨酸间隙的时间进程与反卷积分析

利用3D打印技术来合并MRI与组织学：一个大脑切片协议

在脑血管内皮细胞生物能量功能的评价

脑缺血中风发作时间估计的磁共振成像协议

海马神经元中突触囊泡吞的测量

一种体内评价大鼠缺血性中风模型中血脑屏障的破坏

评估大鼠急性中风后脑恢复的临床前模型

共聚焦显微镜测量肾上腺嗜铬细胞融合孔隙动力学的三种模式

Read Article